Neelesh Kumar

Conference 2022 Pre-recorded Presentation

Video title

Medicinal Plant (Achyranthes aspera): Evaluate their Immunostimulatory Properties feed with Asian Catfish Clarias batrachus L. Fry

Authors and Affiliations

Neelesh Kumar1*, JaiGopal Sharma1, Rina Chakrabarti2

1. Department of Biotechnology, Delhi Technological University, Delhi-110 042 India
2. Department of Zoology, University of Delhi, Delhi-110 007 India

Abstract

Background

Aquaculture makes significant contribution in food security, nutrition and employment generation. The occurrence of infectious diseases is a major challenge to the aquaculture industry as it is affecting sustainable aquaculture. It is essential to improve the fish immunity through various methods as disease free fingerlings will help in boosting production in the field of aquaculture. The effects of numerous medicinal plants such as herbs, spices, herbal medicines, seaweeds etc. have been studied in various aquatic organisms. Achyranthes aspera is a herb and traditional medicinal plant used in treatment of numerous diseases.

Methods

The effect of Immunostimulatory properties and their remedial measures have been studied in Clarias batrachus fry (352.4 ± 7.91 mg). Fish fry were acclimatized for 10 days and then fed with four different diets: 0.5% seeds (D4), 0.5% leaves (D3), 0.25% leaves (D2) of A. aspera and control (D1). After 60 days, all groups were immunized with c-RBC @50 µL/fish. Various samples were collected on 7th, 14th and 21st days after immunization.

Results

The final weight and SGR were significantly higher in D4 diet fed magur compared to other feeding regimes; eventually FCR significantly was higher in D1 diet. Serum lysozyme, myeloperoxidase, nitric oxide synthase and superoxide dismutase levels were significantly higher in D4 diet fed magur after that TBARS and carbonyl protein level were significantly lower in D1 fed magur compared to rest experimental groups. Various immunological and stress related genes were studied.

Conclusions

The present study shows that the inclusion of plant A. aspera in the diets is an alternative approach to replace use of hazardous antibiotics in aquaculture. The plant is found throughout India as a weed, the application of plant ingredients is commercially viable and easily acceptable to the Indian Aquaculturists. The positive results of field study may be beneficial for the Indian aqua farmers. This is an eco-friendly approach for the health management of cultivable fishes.