Samah K. Nasr Eldeen

Conference 2022 Pre-recorded Presentation

Video title

Trehalose enhances the antitumor potential of methotrexate against mice bearing Ehrlich ascites carcinoma

Authors and Affiliations

Mohammed A. El-Magda,*, Abeer Khamisb, Samah K. Nasr Eldeenb, Wafaa M. Ibrahimc, Afrah F. Salamab

1. Anatomy Department, Faculty Veterinary Medicine, Kafrelsheikh University, Post Box 33516, El-Geish Street, Kafrelsheikh, Egypt
2. Chemistry Department, Biochemistry, Faculty of Science, Tanta University, Egypt
3. Medical Biochemistry Department, Faculty of Medicine, Tanta University, Egypt

Abstract

Background

Methotrexate (MTX) is commonly used as a standard chemotherapy for many cancers, however its usage required high doses thereby leading to severe adverse effects. In a trial to
find a suitable neoadjuvant therapy to decrease MTX dosage without lowering its chemotherapeutic efficacy, we investigated the antitumor effect of trehalose (TRE) on mice bearing Ehrlich ascites carcinoma (EAC) and checked whether TRE can enhance the antitumor potential of MTX.

Methods

Swiss albino mice were divided into five groups (25 mice per group)Inoculation of tumor cells on day 0, then Injection of TRE and /or MTX on day 14 then Dissection
and parameters estimation on day 14

Results

Treatment with TRE induced anti-tumor effects against EAC as reveled by a remarkable decrease in body weight, tumor volume, count of viable tumor cells, expression of the anti-apoptotic gene Bcl2 as well as by a significant increase in mean survival time, life
span and expression of the apoptotic gene caspase-3. TRE also caused a significant decrease in autophagic activity of EAC cells as evident by reduction in the expression of the autophagic gene Beclin 1 (Bec1) and the fluorescence intensity of autophagosome marker. Additionally, TRE restored the altered hematological and biochemical parameters and improved the disrupted hepatic tissues of EAC-bearing mice.

Conclusions

Interestingly, co-administration of TRE and MTX showed highest anti-tumor effect against EAC.These data indicate that TRE enhances the antitumor potential of MTX and could be used as neoadjuvant drug to increase the efficacy of the antitumor drug, MTX.