Rabab Kamel

Conference 2024 Poster

Poster Title

Phyto-Emulsomes as a novel nano-carrier for Morine Hydrate to combat leukemia

Authors and Affiliations

Rabab Kamel a , Mona M. AbouSamra a, Sherif M. Afifi b and Asmaa F. Galal c
aPharmaceutical Technology Department, Pharmaceutical and Drug Industries Institute, National Research Centre, Cairo 12622, Egypt.
bPharmacognosy Department, Faculty of Pharmacy, University of Sadat City, Sadat City 32897, Egypt.
cNarcotics, Ergogenics and Poisons Department, Medical Research and Clinical Studies Institute, National Research Centre , Egypt.

Abstract

Background

This study tackles the development of morin hydrate-loaded Phyto-Emulsomes (MH-EMs) using β-sitosterol as a ‘heart-friendly’ alternative to cholesterol for curing leukemia. β-sitosterol was isolated from the aerial parts of C. pallescens Delile (Compositae).

Methods

Phyto-Emulsomes were prepared using different ratios of glyceryl monostearate (GMS), phospholipon 80H (P80H) and ß-sitosterol (SS), then, their size, entrapment efficiency, and drug release were evaluated.

Results

Among the prepared formulae, MH-EM5 composed of GMS: P80H: SS (2:0.5:0.5) had the highest drug release efficiency (RE 82.28 1.96%) and the smallest particle size (271.7 4.86 nm) with a uniform distribution. MH-EM5 was subjected to characterization including the micro-morphological examination using TEM, thermal analysis, in addition to biological evaluation. The biostability of MH-EM5 in serum and bile salts was proved. The potential cytotoxicity of the free drug, MH-EM5 and its corresponding unmedicated formulation against normal oral epithelial cell and acute monocytic leukemia was studied.

Conclusions

The results showed a negligible cytotoxicity in normal cells for all tested samples, while, cancer cells had a significant decrease in the cell viability in case of the selected formula (MH-EM5) compared to the corresponding unmedicated one as well as the free drug, at different concentrations up to 300 μg/ml. Also, the oral bioavailability study proved the
significant increase of all measured pharmacokinetic parameters, after a single oral administration of MH-EM5 compared to the free drug suspension. Regarding these findings, phyto-emulsomes can be considered as a safe and promising nanometric delivery system for the oral administration of morin hydrate due to the enhancement of its solubility, oral bioavailability and antitumor efficacy.