Mujahid Wudil

Conference 2024 Poster

Poster Title

Determination of phytochemicals and Antioxidants activity of n-Hexane leaf extract of moringa oleifera

Authors and Affiliations

Wudil Mujahid Abubakar 1, Dr.Murtala Muhammad 2, Dr.Abdulmumin Yunusa, Dr.Sa’ad 3, Tasiu Abdulmimin Mikail 4

Abstract

Background

Medicinal plants have been used as a natural source of biologically active compounds (Ji et al., 2009; Zhang and Ma, 2018). Some of these biologically active compounds possess beneficial effects, which can be used to improve human health. One of such medicinal plants is Moringa oleifera. M. oleifera is the most most-studied species and most-used species because of its phytochemical and pharmacological properties related to human health.

Methods

One thousand grams (1000g) of the powder Moringa olifeira leave was mixed and soaked in 2000cm3 distilled water in a 2 litre conical flask, the content of the flask and mixed vigorously. The mixture was shaken and top covered with aluminium foil and kept for 48 hours. The aqueous extract obtained by filtration using whatman No1 filter paper and concentrated using vacuum evaporator at 60oC in water bath (OSL200 water bath and shaker Grand instrument, Cambridge). The concentration and total yield of the n-Hexane leave extract of Moringa olifeira will be determined and stored in air tied container for further analysis. Extracts of the plant parts were evaporated to dryness under vacuum at about 45°C using rotary evaporator and further dried to a constant weight at room temperature. A portion of the extract was used for phytochemical analysis for the screening of secondary metabolites. While the antioxidant activity was determine using FRAP assay method.

Results

The phytochemical contents were determined according to the standard methods of analysis while the antioxidant activity was carried out using FRAP. The qualitative analysis showed the presence of Alkaloid, saponins, flavonoid, tannins and total phenols. The concentrations of these phytochemicals in mg/100g were Alkaloids 49 ± 0.30, Saponins 32 ± 0.01, flavonoids 22 ± 0.32, tannins 27 ± 0.32 and total phenols 34 ± 0.01. The antioxidants activity of the leaf extract was evaluated by FRAP. The absorbance of the N-Hexane leaf extract observed under UV-Visible spectroscopy at 593nm and the results obtained was 225.86 ± 0.07 µmol/g while ascorbic acid was used as control with concentration of 254.03 ± 0.05 µmol/g.

Conclusions

Based on the series of studies, it could be concluded that moringa oleifera leaves contain some compounds that are well known for their antioxidant activity and contains secondary metabolites.