Nicholas Awuku Offei

Conference 2022 Poster Presentation

 

Project title

The effect of epigallocatechin -3-gallate on migration in breast cancer cells exposed to staphylococcus aureus peptidoglycan

 

Authors and Affiliations

Nicholas Awuku Offei1

1. University of Ghana

 

Abstract

Background

One of the most commonly diagnosed cancers in women is breast cancer. Diet is regarded to be an important element in breast cancer prevention, and studies indicate that the consumption of green tea reduced the risk of getting breast cancer. Green tea contains EGCG, a polyphenol that has been proven to have anti-tumor properties. Many studies have investigated EGCG on tumor cells, but less is known about EGCG on tumor cells pre- treated with Staphylococcus aureus peptidoglycan (SA-PGN).

Methods

This in vitro research was to examine the cytotoxicity and how it inhibited migration on triple-negative MDA-MB-231 and MDA-MB 468 breast cancer cell lines that had been pre-treated with Staphylococcus aureus peptidoglycan. The MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay was performed to determine cell viability after 72 hours. Cell migration was assessed for the wound healing assay at 0 h, 8 h, 16 h, 24 h, and 48 h.

Results

It was discovered that after 72 hours of exposure to 400 µM EGCG, the cells’ proliferation dramatically decreased, with a lower percentage of cells remaining. The percentage of cells was higher at 50 μM when compared to 400 μM. Both EGCG and SA- PGN slowed the migration rate; however, SA-PGN was more cytotoxic in MDA-MB 231 than in MDA-MB 468. Notably, the motility of the examined breast cancer cells was terminated at the 150 µM EGCG dose.

Conclusions

Our findings indicate that EGCG is a potent growth inhibitor of triple negative breast cancer cell pre-treated with SA- PGN.