Taras V. Zadvornyi

Conference 2022 Poster Presentation

 

Project title

The immunoregulatory genes expression in prostate cancer

 

Authors and Affiliations

Taras Zadvornyi1, Tetiana Borikun1, Natalia Lukianova1

1. R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, NAS of Ukraine, Kyiv, Ukraine

 

Abstract

Background

The involvement of the host immune system in cancer progression is well established. The study aimed to assess the expression of the immunoregulatory genes in prostate cancer (PCa) on protein and mRNA levels

Methods

We studied 75 patients with stage IІ and IІI PCa, who were treated in the National Cancer Institute (Kyiv, Ukraine) in 2015-2017. Morphological and immunohistochemical studies of the expression of the immunoregulatory proteins were performed according to standard protocols. The evaluation of the results was performed using optical microscopy using the H-Score method. The marker (osteopontin (OPN) and lactoferrin (LF)) expression levels were considered to be high at the values from 201 to 300 points of the H-Score, average – from 101 to 200 points of the H-Score, and low at the range from 0 to 100 points of the H-Score. The levels of mRNA SPP1, LTF, and miR-146a, -214 were assessed, using q-PCR. GraphPad Prism 8 software was used to process the results.

Results

According to the distribution of patients according to the criteria of D’Amico al. (1998) in the vast majority of cases (75%) was determined by a low risk of progression of PCa. We found that PCa patients with a high risk of progression of this disease are characterized by a higher level of OPN expression (on 40%, p<0.05) and a significantly lower level of LF expression (on 50%, p<0.05) in tumor tissue, compared with similar patients with low-risk progression. The difference of mRNA SPP1 mRNA LTF expression in patients with a low and high risk of progression of PCa were significantly different (p<0.05). Furthermore, the level of miR-146a and -214 was significantly higher in patients with a high risk of progression of PCa.

Conclusions

Different expressions of the immunoregulatory genes SPP1 and LTF in PCa cells associated with clinical and pathological features of PCa indicate the need for further research aimed at defining the role of these proteins in the pathogenesis of prostate cancer.