Conference 2021 Pre-Recorded Video

 

Project title

Alterations of Costimulatory Molecule Expressions of Mesenchymal Stem Cells That Preconditioned With IFN-γ, IL-4 and IL-10

 

Authors and Affiliations

Alper Tunga Özdemir1, Mustafa Öztatlıcı2, Rabia Bilge Özgül Özdemir3, Büşra Çakır4, Kemal Özbilgin2, Ertan Darıverenli5, Cengiz Kırmaz6

1. Merkezefendi State Hospital, Medical Biochemistry Laboratory, Manisa, Turkey
2. Manisa Celal Bayar University, Medical Faculty, Department of Histology and Embryology, Manisa, Turkey
3. Manisa City Hospital, Department of Allergy and Clinical Immunology, Manisa, Turkey
4. Ege University, Faculty of Engineering, Bioengineering Department, Izmir, Turkey
5. Manisa Celal Bayar University, Medical Faculty, Department of Pharmacology, Manisa, Turkey
6. Manisa Celal Bayar University, Medical Faculty, Department of Allergy and Clinical Immunology, Manisa, Turkey

 

Abstract

Background

Mesenchymal stem cells (MSCs) are strong immunomodulatory cells, and co-stimulation plays an important role in increasing the effects of MSCs on adaptive immune cells. Preconditioning is an approach that increases the effectiveness of MSCs. In this study, it was aimed to investigate the alterations in the costimulatory molecule expressions of MSCs preconditioned with inflammatory cytokines.

Methods

To achieve the aim, MSCs were preconditioned with IFN-γ, IL-4 and IL-10, and the alterations in CD80, CD86, CD137L, CD252, CD274, CD275, and HLA class I, II expressions were analyzed by flow cytometry and qPCR methods. For comparison, the THP-1 macrophages that preconditioned under the same conditions were evaluated as controls.

Results

We found that the HLA-II and costimulatory ligand expressions of MSCs were significantly lower than THP-1 macrophages by either method, but there was no significant difference for HLA-I. When we compared the expression levels of the genes we evaluated in this study, we observed that the expression of MSCs was considerably lower than THP-1 cells.

Conclusions

According to our data, although the MSCs are potent immunomodulatory cells, antigen presentation capabilities are too low to compare to a professional antigen presenting cells. In addition, the costimulatory molecule expressions of MSCs may not alter significantly with the preconditioning approach. However, transfer of costimulatory molecules through gene engineering may enable MSCs to gain a more effective and lasting tolerance potential.