Conference 2021 Pre-Recorded Video


Project title

Effect of water stress on germination of mutant genotypes of groundnut (Arachis hypogaea L.)


Authors and Affiliations

Imane Saibari1,2, Ahlam Hamim2, Mohamed Mohib2, Saïd Barrijal1

1. Biotechnological Valorization Team for Microorganisms Genomics and Bioinformatics, Department of Life Sciences, University of Abdelmalek Essaadi, Tangier, Morocco
2. National Institute for Agronomical Research (INRA), Regional Center of Tangier, Morocco




Drought has emerged strongly in recent decades as a structural element of climate. Morocco is currently experiencing the longest dry episode in its contemporary history, characterized by decreased in precipitation and a clear trend of rising temperatures. On the other hand, farmers face huge challenges due to population growth, climate change and pressure on farmland, which is what makes cultivated plants are exposed to several environmental stresses, there affecting crops productivity. To face these challenges, plant breeding programs was carrying on strategies to develop improved varieties. Therefore this study was conducted to assess the germination and growth, of generation irradiated of groundnut seeds by gamma rays under water stress.


Seeds of two mutant genotypes issue of three degree of gamma irradiation (100, 150 and 200 Gy) of groundnut (Arachis hypogaea ssp. fastigiata), variety KP29 and Fleur 11 type Valencia and Spanish respectively. Our experiments, was carried out on the germination parameter, and growth of seedlings of each mutant genotype in water stress simulated by Polyethylene glycol 4000 (PEG4000) (10%, 20% and 30%).The parameters studied are Germination percentage (GP %), Germination rate (GR), Roots, hypocotyls, and the epicotyls, lengths.


The rate of germination depends on the concentration of PEG used this has been proved by the germination kinetics curves which have shown a very remarkable stress effect at the high level of concentration In general the rate and the time of germination are decreasing for the three tested genotypes of the two varieties except for Feur11 (100Gy) which records a higher than control rate in all three concentrations.


This study showed sensitivity to water stress; however some genotypes tolerate it. These preliminary results clearly show that irradiation with gamma rays can lead to an improvement in the tolerance of peanut to abiotic stress.