Benameur Qada
Algeria
Synergistic effect of Arthemisia herba alba and Thymus vulgaris essential oils against some pathogenic bacteria
Benameur Qada1, Eleonora Di Salvo2, Boutaiba Benklaouz Meki3, Rechidi-Sidhoum Nadra4, Arabi Abed5, Kassous Tahar6, Gervasi Teresa2
1. Département d’Agronomie, Faculté des Sciences de la Nature et de la Vie, Université Abdelhamid Ibn Badis Mostaganem, 27000 Mostaganem, Algeria.
2. Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy.
3. Veterinary Sciences Institute, University of Tiaret, 14000 Tiaret, Algeria.
4. Département des Sciences Alimentaires, Faculté des Sciences de la Nature et de la Vie, Université Abdelhamid Ibn Badis de Mostaganem, Mostaganem, Algeria.
5. Département de Biologie, Faculté des Sciences de la Nature et de la Vie, Université Abdelhamid Ibn Badis de Mostaganem, Mostaganem, Algeria.
6. Mostaganem Food Poultry, Mostaganem, Algeria.
Abstract
Background
With the increasing prevalence of multidrug resistant strains of common bacterial pathogens, the alternative strategies such as the use of medicinal and aromatic plants essential oils (EOs) have been considered for disease control. The present study aimed to evaluate the in vitro antibacterial activity of Thymus vulgaris EO (TVEO) and Artemisia herba-alba EO (AHAEO) against some pathogenic bacteria. The assessment of the synergistic effect between the EO of the two plants was also investigated.
Methods
A number of pathogenic bacterial strains (Escherichia coli, Klebsiella aerogenes, Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis and pseudomonas aeruginosa) were selected to carry out this study. Disc diffusion method and broth micro-dilution assay were conducted to evaluate the antibacterial activity of the EOs. Checkerboard test was used to evaluate the synergistic effect between TVEO and AHAEO.
Results
The results showed that TVEO and AHAEO have a potent activity against all the tested pathogenic bacterial isolates with inhibition zone values in the range of 8 to 45 mm and 7 to 21 mm for TVEO and AHAEO, respectively. The MIC values of TVEO ranged from 4 µg/mL to 8 µg/mL and of AHAEO from 32 µg/mL to 64 µg/mL. Moreover, a synergistic effect, demonstrated by the Fractional Inhibitory Concentration Index, has been detected between the two EOs against all the tested strains.
Conclusions
The current study allowed concluding that both EOs showed not only satisfactory antibacterial activity but also a synergistic effect between the two tested EOs against all the tested pathogenic bacterial strains.
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