Bill C Aglomasa

Conference 2023 Live Talk

Talk title

Secondary bacterial infections in lymphatic filariasis patients: implications of multi-drug resistance

Authors and Affiliations

Bill Clinton Aglomasa1,2*, Cynthia Kyerewaa Adu‑Asiamah2,3, Samuel Opoku Asiedu2, Priscilla Kini2,4, Emmanuel Kobla Atsu Amewu2, Kennedy Gyau Boahen3, Solomon Wireko4,5, Kingsley Isaac Amponsah6, Yaw Duah Boakye1, Vivian Etsiapa Boamah1, Alexander Kwarteng2,4

1. Department of Pharmaceutics, College of Health Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
2. Kumasi Centre for Collaborative Research in Tropical Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
3. Department of Clinical Microbiology, School of Medical Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
4. Department of Biochemistry and Biotechnology, College of Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana
5. Department of Laboratory Technology, Kumasi Technical University, Kumasi, Ghana
6. Department of Pharmacognosy, College of Health Science, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

Abstract

Background

BACKGROUND: Lymphatic filariasis (LF) is associated with the poor living in the tropics of the world. LF patients are at increased risk of developing acute dermatolymphangioadenitis (ADLA), which exposes them to bacterial infections. Secondary bacterial infections (multi-drug resistant) in immunocompromised individuals worsen their prognosis. This has necessitated the resurgence of the One Health approach in tackling priority pathogens.

Methods

METHOD: In this study, bacteria (Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa) were isolated from sixty LF patients and mecA, extended-spectrum beta-lactamase (blaSHV, blaTEM and blaCTX-M) profiles were determined for multi-drug resistant isolates using disk-diffusion, microdilution and conventional polymerase chain reaction (PCR).

Results

RESULTS: The isolates exhibited phenotypic resistance against commonly used beta-lactams that include penicillin, amoxicillin and ampicillin. PCR results confirmed the presence of mecA in all the methicillin-resistant Staphylococcus aureus and blaCTX-M was found in Escherichia coli. The isolates are beginning to show selective pressure for the antibiotics that patients use in their management.

Conclusions

CONCLUSIONS: This study corroborates the ubiquitous nature of antibiotic resistant genes and the selective pressure it is having on bacteria. The is alarming, especially in immunocompromised patients and there is the need to factor these findings in morbidity management of LF patients.