Matthew O Oki

Conference 2023 Presentation

 

Project title

Isolation, Identification and Antibiotic Susceptibility Profile of Bacterial Isolated from fomites in some selected Daycare centers in Ile-Ife Nigeria

 

Authors and Affiliations

Matthew Oki1, Anthonia O. Oluduro1,

1. Department of Microbiology, Obafemi Awolowo University, Ile-Ife Nigeria.

 

Abstract

Background

Inanimate objects are known carrier of infectious agent and means by which these agents can be transmitted to human and other parts of the body, these inanimate objects are referred to as Fomites. Most of the outbreaks of infections are caused by direct contact with these Fomites such as toys, chairs, tables, diaper changing area, bed etc. The study reported the isolation, identification and antibiotic susceptibility profile of bacteria cultured from Fomites in some selected Day care centers in Ile-Ife.

Methods

Samples were collected from fomites in five different day care centers in ile-ife to determine the type of microorganisms present. Some of the fomites selected and from which samples are collected includes toys, bed, Tables, etc. The samples were collected by using a sterile swab stick to swab the surface of the fomites and transported back into the laboratory for further analysis. Different media were used in the identification of contaminating bacteria and 43 isolates were recovered. Morphological characteristics of these isolates were noted, gram’s staining, microscopic examination and different biochemical test were carried out to identify the bacteria types. Antibiotic susceptibility test was also carried out to determine the susceptibility of the bacteria isolates to different antibiotics using the disc diffusion techniques.

Results

Out of the 43 bacteria isolated, 35 were Gram positive while the remaining 8 were Gram negative bacteria. The Bacteria isolated in cludes; Bacillus species (48.84%), Corynebacterium xerosis (25.58%), Enterobacteriaceae (16.28%), Staphylococcus aureus (4.65%), Corynebacterium kutsceri (2.33%), and Pseudomonas aeruginosa (2.33%). All the Gram positive isolates except Corynebacterium species were 100% susceptible to ciprofloxacin and gentamycin. Bacillus species were 4.76% susceptible to cefuroxime, ampicillin and augmentin of 21 isolates. Corynebacterium species were 91.67% susceptible to gentamycin, ciprofloxacin and vancomycin and 8.33% susceptible to ampicillin of 12 isolates. Staphylococcus aureus were 100% resistant to ampicillin, erythromycin and augmentin. All the Gram negative isolates were 100% susceptible to ciprofloxacin and amikacin.

Conclusions

The study shows that fomites from day care centers are usually contaminated with bacteria, majority of which constitute the normal flora of the human body and are opportunistic in nature which can cause opportunistic infection. It is therefore advisable that proper hygienic practices should be done before and after coming in contact with these fomites also behavioral patterns such as putting hands or toys in the mouth and careless playing with fomites such as pencil in day care centers by these little children should be prevented to avoid the cause of an opportunistic infection. Also workers taking care of the children should ensure proper hygiene such as washing of hands before and after taking care of the children to reduce the transfer of bacteria.