Silas Onyango Awuor

Conference 2023 Presentation

 

Project title

Antibiotic Susceptibility Pattern, antibiofilm activity and virulence factors of Bacterial Isolates from swab samples at Kenyan hospital.

 

Authors and Affiliations

Silas O. Awuor1*, Richard Mariita2, Eric O. Omwenga 3, Jonathan Musila4`

1. Jaramogi Oginga Odinga Teaching and Referral Hospital-Microbiology Department, P.O Box 849, Kisumu, Kenya.
2. Microbial BioSolutions, Troy, New York, 12180 USA
3. Kisii University, School of Health Sciences, P.O. Box 408 – 40200, Kisii, Kenya
4. Fayetteville State University, NC 28301

 

Abstract

Background

The extensive uses of antibiotics, together with the span of time over which they have been available, have led to the development of resistant bacterial pathogens contributing to morbidity and mortality. This study was aimed to evaluate bacterial isolates and their drug susceptibility patterns from inpatients and outpatients with pus and/or wound discharge.

Methods

A cross sectional study was conducted at the Jaramogi Oginga Odinga Teaching Referral Hospital from May to August, 2022.Swab samples were collected and inoculated into appropriate media. The bacterial pathogens were identified using standard microbiological methods. Antimicrobial susceptibility tests were performed using disk diffusion technique method.

Results

A total of 135 study samples were included in this study with positive bacterial isolation rate of 73 (54.1%). Of all, 79 (59%) were males. Thirty-seven (27.6%) of the isolates were Gram-positive and 36 (26.9%) were Gram-negative. From the total isolates, Staphylococcus aureus was the most predominant isolate 28 (20.7%) followed by Klebsiella species 20 (14,8%), Pseudomonas aeruginosa 10 (7.4%), coagulase negative staphylococci spp. 9 (6.7%) and lastly E. coli 6 (4.4%). Gram-positive isolates were resistant to cotrimoxazole 16 (21.9%), clindamycin and Erythromycin at 8(11%) while Gram-negative isolates were resistant to ampicillin 14 (19.2%) followed by gentamycin 11 (15.1%), imipenem 8 (11%), cefoxitin and cotrimoxazole at 5 (6.8%) each, amikacin, cefepime and ciprofloxacin at 4 (5.5%) each and lastly cefuroxime at 2 (2.7%) of the total 73 positive isolates.

Conclusions

High prevalence of bacterial isolates was found, Staphylococcus aureus being the most dominant. High rates of multiple drug resistance pathogens to the commonly used antimicrobial agents were isolated. Therefore, AMR team of the facility should properly monitor the choice of antibiotics to be used as prophylaxis and empiric treatment in the study are