Kiran Kishor Khandare

Conference 2023 Presentation

Project title

SFE-LS optimized extract inhibits iNOS, IL-6 and IL1β and STAT1 STAT3 inflammatory proteins.

Authors and Affiliations

Kiran1, Saswata Goswami1

1. Chemical engineering division, Center of Innovative and Applied Bioprocessing

Abstract

Background

Lagerstroemia speciosa (Banaba) is a underexplored plant that is rich source of flavonoids. Flavonoids are bioactive compounds that are well known to have potent immunomodulatory effects. Suppression of the proteins that persistently activate the inflammatory response in macrophages is an important target for the treatment of various chronic diseases. The current study was designed to evaluate the effects of Lagerstroemia speciosa supercritical fluid extract (SFE-LS) on bacterial lipopolysaccharide (LPS)-induced inflammatory responses in RAW 264.7 murine macrophages.

Methods

The SFE-LS of flavonoids was optimized using a central composite design. The compounds in the extracts were confirmed using gas chromatography and mass spectroscopy. Findings of inhibitory activity of iNOS, IL-6, IL1, STAT1, and STAT3 in 264.7 lines are confirmed with the help of literature survey.

Results

MTT assay results indicated that SFE-LS is non-toxic to RAW 264.7 cells up to a maximum assayed concentration of 40 μg/mL. The SFE-LS inhibits LPS-induced NO production and downregulates iNOS expression in a concentration-dependent manner. Furthermore, the extract inhibited LPS-induced expression of interleukin-6 (IL-6) and interleukin-1 (IL-1). Mechanistic studies revealed that PDN-SCFE does not influence the NFκB and MAPK pathways. However, it showed a significant inhibitory effect on LPS-induced activation of STAT1 and STAT3 proteins in macrophages.

Conclusions

The present findings revealed that flavonoids present in the optimized SFE-LS significantly inhibit the proinflammatory mediators iNOS, IL-6 and IL1β and also suppress the inflammatory activators STAT1 AND STAT3