Samah Kotb Nasr Eldeen

Conference 2023 Presentation

Project title

Knockdown of CD-74 in the Proliferative and Apoptotic Activity of Breast Cancer Cells

Authors and Affiliations

Samah K. Nasr Eldeen, Hussain Al Ssadh, Waleed Al Abdulmonem, Zafar Rasheed, Inamul Hasan Madar, Jamila Alhoderi, Ali Alradhwan, Noura Alasmael, Abdullah Alkhamiss, Nelson Fernández

Abstract

Background

The cluster of differentiation (CD) 74 is known for its immunological functions and its elevated level was reported in various cancer cells.The aim of the present study was to investigate the expression and potential roles of CD74 in the proliferative and apoptotic activity of breast cancer.

Methods

Expression of CD74, macrophage migration inhibitory factor (MIF) and CD44 was assayed in CAMA-1 and MDA-MB-231 cell lines using flow cytometry. CD74 was knocked down using CD74 siRNA-transfection in CAMA-1, and MDA-MB-231 cells and proliferation and apoptosis were determined in the transfected breast cancer cells.

Results

Flow cytometric analysis for cell surface expression of CD74, MIF and CD44 in the breast cancer cells displayed; Empty histograms represented the expression of CD74, MIF, and CD44; Expression in Raji, Jurkat, and HeLa cells are used positive controls. Whereas, grey-filled histograms were shown as a negative control obtained from isotype-matched with control antibody; Colocalization of CD74 and MIF on the cell-surface of CAMA-1 and MDA-MB-231 cells, determined by confocal microscopy analysis.
CD74 knockdown by CD74 siRNA transfection reagent in CAMA-1 and MDA-MB-231 cells;

Conclusions

We concluded that the interactions of CD74 with MIF and CD74 with CD44 could be a potential tumour marker for breast cancer cells. Moreover, the level of co-expression of MIF and CD74 or CD44 could be a surrogate marker for the efficacy of anti-angiogenic drugs, particularly in breast cancer tumours. In short, the study revealed the potential roles of CD74 in the proliferation and apoptosis of breast cancer which may serve as a potential therapeutic target for breast cancer.