Conference 2021 Poster Presentation

 

Project title

Physiochemical characterization of cyanobacteria at different growth stages screened from diverse region of India

 

Authors and Affiliations

Roshan Kumar1

1. Department of Human Genetics & Molecular Medicine, Central University of Punjab, Bathinda, India-151001

 

Abstract

Background

Cyanobacteria (blue green algae) are prokaryotic organisms that exhibit the general characteristics of gram-negative bacteria. These are morphologically, physiologically and developmentally diverse and are unique due to their capacity to carry out oxygenic photosynthesis. Some of them also have the capacity to fix atmospheric nitrogen. Potential of cyanobacteria as a source of food, feed, natural colors, biofuel, biofertilizer, enzymes, pharmaceuticals and other fine chemicals is well recognized.

Methods

Soil samples were collected from different part of, India, These were subjected to enrichment culture technique using BG-11 medium (Stanier et al. 1971) in a culture room at 52-55μmole photon/m2/s light intensity with 16/8 light and dark period and 28±.2OC temperature. Isolated and purified strains were studied microscopically and the taxonomic identification upto generic level was done following the keys given by Desikachary (1959). Homogenous samples were taken during different growth phase at 7, 14, 21, 28 and 35 days for estimation of chlorophyll, carotenoids, carbohydrate, proteins, nitrogenase reductase (NR) and glutamine synthetase (GS) in cyanobacterial (20) strains belonging to different genera.

Results

The Cyanobacterial isolates showed wide variation among each other with respect to growth and production of carbohydrates, total soluble proteins, carotenoids, NR and GS. Growth in term of total chlorophyll increased gradually and maximum chlorophyll was expressed at 35th days of incubation where as maximum carbohydrates, total soluble proteins, carotenoids, NR and GS were produced at 14th day and decreased gradually thereafter. Of the 20 cyanobacterial strains, Phormidium sp. (SP18) was the promising strain among all as far as the

Conclusions

Cyanobacteria have a large history of existence and use but their transition from biology to biotechnology has been triggered off recently. Beside its contribution in agriculture which is well documented these comprise a vast potential resource for the production of bioactive compounds, food supplement for human, aquaculture feed, fine chemicals, renewable fuels and environmental application etc. The present study has shown that there exists immense biochemical diversity among these organisms and this can be exploited in many ways for the benefit of mankind.

 


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