Conference 2021 Poster Presentation

Project title

In silico characterization and sequence analysis of Toll like receptor 1 gene of camel

Authors and Affiliations

Dhirendra Suthar 1,2, Sunil Maherchandani2

1. Gene and Protein Engineering Lab, National Institute of Animal Biotechnology, Hyderabad, Telangana – 500032, India
2. Department of Veterinary Microbiology and Biotechnology, C.V.A.S,Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan- 334001, India

Abstract

Background

Most of the vertebrates have innate immune system as a first line of defense against pathogens. Innate immunity relies a set of germ line encoded receptor known as pattern recognition receptor. These PRR further recognized by pathogen associated molecular pattern which is derived by the various class of pathogen like Virus and Gram positive, negative bacteria. The most common PRR is Toll like Receptor. They are a family of pattern recognition receptors which act as a primary sensor of the innate immune system to recognize microbial pathogens. Camel has acquired many special abilities and attributes to survive in harsh environments and is comparatively resilient to infections. Till now, no reports are there about the characterization TLR1 gene and its role in virulence mechanism of different pathogens Particularly in camel. Here, we tried to characterize the TLR 1 gene of camel using In-silico approaches at genomic and proteomic level.

Methods

The different overlapping fragments of camel TLR1 cDNA obtained by PCR were sequenced and assembled into a 2430 bp long contig. The sequence was submitted to NCBI (GenBank accession no. MG655186). ORF of camel TLR1 was translated into 796 amino acid long protein sequence. The same number of amino acids was reported in pig and rabbit. The camel TLR-1 amino acid sequence was also compared with TLR-1 of different species. We noticed that highest similarity of camel TLR1 with TLR1 of pig, whereas lowest similarity was found with that of rabbit. Further, variation of two amino acids was recorded between encoded polypeptide sequence of camel TLR1 and predicted polypeptide sequence of camel TLR-1 at position 506 and 583. The Phylogenetic tree was constructed using amino acid translated from coding sequence of TLR1. These findings suggest the closest phylogenetic relationship of camel TLR1 gene with pig and distantly related to rabbit TLR-1.

Results

In this study we attempt to characterized TLR 1 coding sequence of camel. The camel TLR 1 encodes 2391 long nucleotide open reading frame, translated into 796 aa. The 3D structure of camel TLR 1 amino acids is common to TLR 1 of other vertebrates. The camel TLR 1 has 7 LRR domains which are similar to that of goat. Higher number of LRRs indicate its crucial role in the innate immune defense system as it can recognize comparatively more number of PAMPs.